骆池怡;彭楚芳;杨媛;秦满;王媛媛;

• 1:北京大学口腔医学院·口腔医院儿童口腔科国家口腔疾病临床医学研究中心口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室

摘要(Abstract)：

目的:比较Clearfil SE Bond(SE)、Adper~(TM)Easy One(EO)、Scotchbond Universal(SBU) 3种自酸蚀粘接系统对乳牙釉质和牙本质粘接耐久性的影响,以及粘接界面轻度唾液污染后即刻吹干对粘接耐久性的影响。方法:将240个乳牙釉质及240个牙本质样本随机分为16组(n=15个/组),实验组选用SE、EO、SBU等3种不同自酸蚀粘接系统,对照组选用Adper~(TM)Single Bond Plus(SL)全酸蚀粘接系统,在无污染或有唾液污染、蒸馏水储存(水浴储存24 h)或水浴循环老化(5℃和55℃水浴中循环5 000次)等两种不同储存条件下,测量每组中12个试样的剪切粘接强度,用扫描电子显微镜观察分析剩余3个试样的粘接界面情况。采用三因素方差分析法和Tukey检验对数据进行统计学分析。结果:对于乳牙牙釉质粘接,全酸蚀粘接剂的即刻剪切粘接强度(28.92±1.83) MPa和老化后剪切粘接强度(27.27±3.03) MPa均显著高于其他组,差异有统计学意义(P <0.01); EO组有唾液污染时即刻剪切粘接强度(11.88±3.17) MPa或老化后剪切粘接强度(11.90±3.98) MPa均显著低于其无唾液污染时的即刻剪切粘接强度(19.57±3.89) MPa或老化后剪切粘接强度(19.01±5.03) MPa,差异有统计学意义(P <0.01)。对于乳牙牙本质的粘接,老化处理后全酸蚀粘接剂的剪切粘接强度(14.31±1.97) MPa显著低于其他粘接剂组,差异有统计学意义(P <0.01); EO组有唾液污染时的即刻剪切粘接强度(12.99±2.66) MPa显著低于其无唾液污染时剪切粘接强度(18.63±3.61) MPa,差异有统计学意义(P <0.01); EO组的无/有污染状态下的剪切粘接强度[(14.41±2.68) MPa和(10.93±2.18) MPa]均显著低于SE组[(21.10±4.40) MPa和(19.56±3.64) MPa]和SBU组[(22.27±5.43) MPa和(20.60±5.11) MPa],差异有统计学意义(P <0.01)。结论:全酸蚀粘接剂对乳牙釉质粘接耐久性更佳; SE和SBU对乳牙牙本质有更好的粘接耐久性;粘接界面轻度唾液污染后即刻吹干处理可恢复SE和SBU的粘接强度,但对EO的粘接性能影响较大,无法恢复其粘接耐久性。

关键词(KeyWords)： 乳牙;牙釉质;牙本质;自酸蚀;唾液污染

Abstract:

Keywords:

基金项目(Foundation): 中华口腔医学会青年临床科研基金牙科粘接技术研究项目(CSA-B2018-10)~~

作者(Author): 骆池怡;彭楚芳;杨媛;秦满;王媛媛;

Email:

DOI: 10.19723/j.issn.1671-167X.2021.01.008

参考文献(References)：

• [1]Perdigao J,Sezinando A,Monteiro PC.Laboratory bonding ability of a multi-purpose dentin adhesive[J].Am J Dent,2012,25(3):153-158．
• [2]Munoz MA,Luque-Martinez IV,Malaquias P,et al.In vitro longevity of bonding properties of universal adhesives to dentin[J].Oper Dent,2015,40(3):282-292．
• [3]Lawson NC,Robles A,Fu CC,et al.Two-year clinical trial of a universal adhesive in total-etch and self-etch mode in non-carious cervical lesions[J].J Dent,2015,43(10):1229-1234．
• [4]Vermelho PM,Reis AF,Ambrosano GMB,et al.Adhesion of multimode adhesives to enamel and dentin after one year of water storage[J].Clinical Oral Investigations,2016,21 (5):1707-1715．
• [5]Santschi K,Peutzfeldt A,Lussi A,et al.Effect of salivary contamination and decontamination on bond strength of two one-step selfetching adhesives to dentin of primary and permanent teeth[J].J Adhes Dent,2015,17(1):51-57．
• [6]Soares FZM,Rocha Rd O,Raggio DP,et al.Microtensile bond strength of different adhesive systems to primary and permanent dentin[J].Pediatr Dent,2005,27(6):457-462．
• [7]Ozmen B,Koyuturk AE,Tokay U,et al.Evaluation of bond strength of self-etching adhesives having different p H on primary and permanent teeth dentin[J].J Appl Biomater Funct Mater,2015,13(3):e274-e279．
• [8]Osorio R,Yamauti M,Ruiz-Requena ME,et al.MMPs activity and bond strength in deciduous dentine-resin bonded interfaces[J].JDent,2013,41(6):549-555．
• [9]Soares FZ,Lenzi TL,de Oliveira Rocha R.Degradation of resindentine bond of different adhesive systems to primary and permanent dentine[J].Eur Arch Paediatr Dent,2017,18 (2):113-118．
• [10]Yaguchi T.Layering mechanism of MDP-Ca salt produced in demineralization of enamel and dentin apatite[J].Dent Mater,2017,33(1):23-32．
• [11]Kulkarni AS,Kokate S,hegde V,et al.The effect of saliva contamination on shear bond strength of two universal bonding agents:an in vitro study[J].J Clin Diagn Res,2018,12(4):6-10．
• [12]Cobanoglu N,Unlu N,Ozer F,et al.Bond strength of self-etch adhesives after saliva contamination at different application steps[J].Oper Dent,2013,38(5):505-511．
• [13]Loguercio AD,Munoz MA,Luque-Martinez I,et al.Does active application of universal adhesives to enamel in self-etch mode improve their performance?[J].J Dent,2015,43 (9):1060-1070．
• [14]Cardenas AFM,Siqueira FSF,Bandeca MC,et al.Impact of p Hand application time of meta-phosphoric acid on resin-enamel and resin-dentin bonding[J].J Mech Behav Biomed Mater,2018,78:352-361．
• [15]Gong HH,Guo XW,Cao DF,et al.Photopolymerizable and moisture-curable polyurethanes for dental adhesive applications to increase restoration durability[J].J Mater Chem B,2019,7(5):744-754．
• [16]Antoniazzi BF,Nicoloso GF,Lenzi TL,et al.Selective acid etching improves the bond strength of universal adhesive to sound and demineralized enamel of primary teeth[J].J Adhes Dent,2016,18(4):311-316．
• [17]Memarpour M,Shafiei F,Razmjouei F,et al.Shear bond strength and scanning electron microscopy characteristics of universal adhesive in primary tooth dentin:An in vitro study.[J].Dent Res J(Isfahan),2018,15(4):264-270．
• [18]Porto IM,Saiani RA,Chan KL,et al.Organic and inorganic content of fluorotic rat incisors measured by FTIR spectros-copy[J].Spectrochim Acta A Mol Biomol Spectrosc,2010,77 (1):59-63．
• [19]Silverstone LM,Saxton CA,Dogon IL,et al.Variation in the pattern of acid etching of human dental enamel examined by scanning electron microscopy[J].Caries Res,1975,9 (5):373-387．
• [20]Sumikawa DA,Marshall GW,Gee L,et al.Microstructure of primary tooth dentin[J].Pediatr Dent,1999,21 (7):439-444．
• [21]Lenzi TL,Guglielmi Cde A,Arana-Chavez VE,et al.Tubule density and diameter in coronal dentin from primary and permanent human teeth[J].Microsc Microanal,2013,19 (6):1445-1449．
• [22]Ye Q,Wang Y,Spencer P.Nanophase separation of polymers exposed to simulated bonding conditions[J].J Biomed Mater Res Part B Appl Biomater,2009,88(2):339-348．
• [23]Werle SB,Steglich A,Soares FZM,et al.Effect of prolonged air drying on the bond strength of adhesive systems to dentin.[J].Gen Dent,2015,63(6):68-72．
• [24]Landuyt KLV,Snauwaert J,Munck JD,et al.Origin of interfacial droplets with one-step adhesives[J].J Dent Res,2007,86(8):739-744．
• [25]Pragasam AX,Duraisamy V,Nayak UA,et al.Evaluation of sealing ability two self-etching adhesive systems and a glass ionomer lining LC under composite restoration in primary tooth:An in vitro study[J].J Pharm Bioallied Sci,2015,7 (Suppl 2):518-523．
• [26]Angker L,Nockolds C,Swain MV,et al.Quantitative analysis of the mineral content of sound and carious primary dentine using BSE imaging[J].Arch Oral Biol,2004,49(2):99-107．
• [27]van Meerbeek B,de Munck J,Yoshida Y,et al.Buonocore memorial lecture adhesion to enamel and dentin:current status and future challenges[J].Oper Dent,2003,28(3):215-235.